CoraLite® Plus 488-conjugated DNM1 Monoclonal antibody

DNM1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Rabbit, Pig, Chicken

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2A11

Cat no : CL488-68009

Synonyms

dynamin 1, Dynamin-1, Dynamin1, Dynamin I, DNM 1

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Validation Data Gallery

• 

  FC experiment of HeLa using CL488-68009

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DNM1 (CL488-68009, Clone:1B2A11) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68009 targets DNM1 in FC (Intra) applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig, Chicken samples.

• Tested Reactivity: Human, Rat, Mouse, Rabbit, Pig, Chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNM1 fusion protein Ag12932
• Full Name: dynamin 1
• Calculated Molecular Weight: 851 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC063850
• Gene Symbol: DNM1
• Gene ID (NCBI): 1759
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C