pig brain tissue were subjected to SDS PAGE followed by western blot with 68009-1-Ig (DNM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
pig brain tissue were subjected to SDS PAGE followed by western blot with 68009-1-Ig (DNM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 68009-1-Ig
WB result of DNM1 antibody (68009-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNM1 transfected A549 cells.
WB result of DNM1 antibody (68009-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNM1 transfected A549 cells.
IHC staining of rat brain using 68009-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 68009-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Neuro-2a using 68009-1-Ig
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using DNM1 antibody (68009-1-Ig, Clone: 1B2A11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using DNM1 antibody (68009-1-Ig, Clone: 1B2A11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Neuro-2a cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:20000-1:100000
Immunohistochemistry (IHC)
IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68009-1-Ig targets DNM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig, Chicken samples.
pig brain tissue were subjected to SDS PAGE followed by western blot with 68009-1-Ig (DNM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 68009-1-Ig
WB result of DNM1 antibody (68009-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNM1 transfected A549 cells.
IHC Figures
IHC staining of rat brain using 68009-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 68009-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of Neuro-2a using 68009-1-Ig
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using DNM1 antibody (68009-1-Ig, Clone: 1B2A11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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