DHCR24 Monoclonal antibody, PBS Only

DHCR24 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6E11

Cat no : 60028-1-PBS

Synonyms

DHCR24, Diminuto/dwarf1 homolog, Seladin 1

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Validation Data Gallery

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  WB analysis using 60028-1-Ig (same clone as 60028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60028-1-Ig (same clone as 60028-1-PBS)

  WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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Product Information

60028-1-PBS targets DHCR24 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DHCR24 fusion protein Ag0756
• Full Name: 24-dehydrocholesterol reductase
• Calculated Molecular Weight: 60 kDa, 516 aa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC004375
• Gene Symbol: DHCR24
• Gene ID (NCBI): 1718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).