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DHCR24 Monoclonal antibody

DHCR24 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6E11

Cat no : 60028-1-Ig

Synonyms

DHCR24, Diminuto/dwarf1 homolog, Seladin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHCR24 Monoclonal antibody (60028-1-Ig)

    WB analysis using 60028-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DHCR24 Monoclonal antibody (60028-1-Ig)

    WB analysis of HepG2 using 60028-1-Ig

    WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DHCR24 Monoclonal antibody (60028-1-Ig)

    IHC staining of human prostate cancer using 60028-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DHCR24 Monoclonal antibody (60028-1-Ig)

    IHC staining of human prostate cancer using 60028-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60028-1-Ig targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHCR24 fusion protein Ag0756
Full Name 24-dehydrocholesterol reductase
Calculated Molecular Weight 60 kDa, 516 aa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC004375
Gene Symbol DHCR24
Gene ID (NCBI) 1718
RRIDAB_874115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Protocols

Product Specific Protocols
WB protocol for DHCR24 antibody 60028-1-IgDownload protocol
IHC protocol for DHCR24 antibody 60028-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols