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CoraLite® Plus 488-conjugated DGKA Polyclonal antibody

DGKA Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11547

Synonyms

EC:2.7.1.107, DGK-alpha, DGK alpha, DAGK1, DAGK



Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11547 targets DGKA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKA fusion protein Ag2075
Full Name diacylglycerol kinase, alpha 80kDa
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023523
Gene Symbol DGKA
Gene ID (NCBI) 1606
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
FC protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
Standard Protocols
Click here to view our Standard Protocols