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  • KD/KO Validated

DGKA Polyclonal antibody

DGKA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11547-1-AP

Synonyms

EC:2.7.1.107, DGK-alpha, DGK alpha, DAGK1, DAGK



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MOLT-4 cells, human cerebellum tissue, HEK-293 cells, mouse cerebellum tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11547-1-AP targets DGKA in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKA fusion protein Ag2075
Full Name diacylglycerol kinase, alpha 80kDa
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023523
Gene Symbol DGKA
Gene ID (NCBI) 1606
RRIDAB_2245857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

Protocols

Product Specific Protocols
WB protocol for DGKA antibody 11547-1-APDownload protocol
IHC protocol for DGKA antibody 11547-1-APDownload protocol
IF protocol for DGKA antibody 11547-1-APDownload protocol
IP protocol for DGKA antibody 11547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.

Authors - David Novo
humanWB

Nat Chem Biol

Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.

Authors - Timothy B Ware
  • KD Validated
humanWB

Nat Commun

Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.

Authors - Christoph Weigel
humanWB

EMBO J

Dysfunctional natural killer cells can be reprogrammed to regain anti-tumor activity

Authors - Batel Sabag
humanWB

Cancer Discov

Diacylglycerol Kinase α Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers.

Authors - Dominguez Charli L CL
  • KD Validated
humanWB,IHC

Clin Cancer Res

DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.

Authors - Jie Li
  • KD Validated