DDX5 Monoclonal antibody, PBS Only

DDX5 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3D2

Cat no : 67025-1-PBS

Synonyms

DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

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Validation Data Gallery

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  WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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  WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67025-1-Ig (same clone as 67025-1-PBS)

  IP result of anti-DDX5 (IP:67025-1-Ig, 5ug; Detection:67025-1-Ig 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67025-1-Ig (same clone as 67025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67025-1-Ig (same clone as 67025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67025-1-Ig (same clone as 67025-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67025-1-Ig (same clone as 67025-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67025-1-Ig (DDX5 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67025-1-PBS targets DDX5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX5 fusion protein Ag24821
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: NM_004396
• Gene Symbol: DDX5
• Gene ID (NCBI): 1655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.