CoraLite® Plus 488-conjugated DCT Monoclonal antibody

DCT Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3D4

Cat no : CL488-68114

Synonyms

TRP 2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, EC:5.3.3.12, DT

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Validation Data Gallery

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  IF Staining of A549 using CL488-68114

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 DCT antibody (CL488-68114, Clone: 1B3D4 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68114 targets DCT in IF/ICC applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DCT fusion protein Ag26165
• Full Name: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Calculated Molecular Weight: 519 aa, 59 kDa
• Observed Molecular Weight: 55-63 kDa
• GenBank Accession Number: BC028311
• Gene Symbol: DCT
• Gene ID (NCBI): 1638
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).