DCT Monoclonal antibody

DCT Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D4

Cat no : 68114-1-Ig

Synonyms

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2

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Validation Data Gallery

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  WB analysis of A375 using 68114-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 68114-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skin using 68114-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 68114-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 68114-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 68114-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HL-60 cells, pig skin tissue, mouse skin tissue, mouse testis tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68114-1-Ig targets DCT in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

• Tested Reactivity: Human, Mouse, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DCT fusion protein Ag26165
• Full Name: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Calculated Molecular Weight: 519 aa, 59 kDa
• Observed Molecular Weight: 55-63 kDa
• GenBank Accession Number: BC028311
• Gene Symbol: DCT
• Gene ID (NCBI): 1638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).