Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody, PBS Only

DC-SIGNR/CD299 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241683G8

Cat no : 98205-1-PBS

Synonyms

CLEC4M, CD299, CD209L1, CD209L, CD209 antigen-like protein 1

---

Validation Data Gallery

• 

  FC experiment of Transfected HEK-293T using 98205-1-RR (same clone as 98205-1-PBS)

  1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (98205-1-RR, Clone: 241683G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98205-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98205-1-RR (same clone as 98205-1-PBS)

  1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (98205-1-RR, Clone:241683G8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98205-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98205-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98205-1-RR against Human DC-SIGNR/CD299 were performed. The affinity constant is 3.68 nM.

---

Product Information

98205-1-PBS targets DC-SIGNR/CD299 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: C-type lectin domain family 4, member M
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC038851
• Gene Symbol: DC-SIGNR/CD299
• Gene ID (NCBI): 10332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Dendritic cell-specific intracellular adhesion molecule-3-grabbing non-integrin (DC-SIGN/CD209) and DC-SIGN related (DC-SIGNR/CD299/L-SIGN/CD209L/CLEC4M) are trans-membrane C-type lectins that are calcium-dependent carbohydrate-binding proteins, which can act as cell-adhesion and pathogen-recognition receptors (PMID: 18458800). DC-SIGN and DC-SIGNR share 77% amino acid identity (PMID: 10975799; 11226297). DC-SIGN is expressed on subsets of dendritic cells and macrophages while DC-SIGNR is restricted to endothelial cells of the liver, lymph nodes and placenta (PMID: 17509452). DC-SIGNR servers as a receptor for ICAM-3. It recognizes a wide range of evolutionarily divergent pathogens.