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Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody

DC-SIGNR/CD299 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241683G8

Cat no : 98205-1-RR

Synonyms

CLEC4M, CD299, CD209L1, CD209L, CD209 antigen-like protein 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antib (98205-1-RR)

    FC experiment of Transfected HEK-293T using 98205-1-RR

    1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (98205-1-RR, Clone: 241683G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antib (98205-1-RR)

    FC experiment of Transfected HEK-293T using 98205-1-RR

    1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (98205-1-RR, Clone:241683G8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98205-1-RR

    Affinity and Kinetic Characterization of 98205-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98205-1-RR against Human DC-SIGNR/CD299 were performed. The affinity constant is 3.68 nM.

Tested Applications

Positive FC detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98205-1-RR targets DC-SIGNR/CD299 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name C-type lectin domain family 4, member M
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC038851
Gene Symbol DC-SIGNR/CD299
Gene ID (NCBI) 10332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Dendritic cell-specific intracellular adhesion molecule-3-grabbing non-integrin (DC-SIGN/CD209) and DC-SIGN related (DC-SIGNR/CD299/L-SIGN/CD209L/CLEC4M) are trans-membrane C-type lectins that are calcium-dependent carbohydrate-binding proteins, which can act as cell-adhesion and pathogen-recognition receptors (PMID: 18458800). DC-SIGN and DC-SIGNR share 77% amino acid identity (PMID: 10975799; 11226297). DC-SIGN is expressed on subsets of dendritic cells and macrophages while DC-SIGNR is restricted to endothelial cells of the liver, lymph nodes and placenta (PMID: 17509452). DC-SIGNR servers as a receptor for ICAM-3. It recognizes a wide range of evolutionarily divergent pathogens.

Protocols

Product Specific Protocols
FC protocol for DC-SIGNR/CD299 antibody 98205-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols