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CoraLite® Plus 488-conjugated DARS Monoclonal antibody

DARS Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C5D3

Cat no : CL488-67930

Synonyms

AspRS, Aspartyl-tRNA synthetase, aspartyl tRNA synthetase, Aspartate--tRNA ligase, cytoplasmic, Aspartate tRNA ligase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated DARS Monoclonal anti (CL488-67930)

    IF Staining of HeLa using CL488-67930

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DARS antibody (CL488-67930, Clone: 3C5D3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DARS Monoclonal anti (CL488-67930)

    FC experiment of HeLa using CL488-67930

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DARS (CL488-67930, Clone:3C5D3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67930 targets DARS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DARS fusion protein Ag7059
Full Name aspartyl-tRNA synthetase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight50-57 kDa
GenBank Accession NumberBC000629
Gene Symbol DARS
Gene ID (NCBI) 1615
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DARS antibody CL488-67930Download protocol
FC protocol for CL Plus 488 DARS antibody CL488-67930Download protocol
Standard Protocols
Click here to view our Standard Protocols