DARS Monoclonal antibody

DARS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C5D3

Cat no : 67930-1-Ig

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

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Validation Data Gallery

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  WB analysis using 67930-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67930-1-Ig (DARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 67930-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67930-1-Ig (DARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67930-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DARS antibody (67930-1-Ig, Clone: 3C5D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67930-1-Ig targets DARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DARS fusion protein Ag7059
• Full Name: aspartyl-tRNA synthetase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC000629
• Gene Symbol: DARS
• Gene ID (NCBI): 1615
• RRID: AB_2918682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.