DARS Monoclonal antibody, PBS Only

DARS Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C5D3

Cat no : 67930-1-PBS

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

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Validation Data Gallery

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  WB analysis using 67930-1-Ig (same clone as 67930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67930-1-Ig (DARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67930-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67930-1-Ig (same clone as 67930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67930-1-Ig (DARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67930-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67930-1-Ig (same clone as 67930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DARS antibody (67930-1-Ig, Clone: 3C5D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67930-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67930-1-PBS targets DARS in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DARS fusion protein Ag7059
• Full Name: aspartyl-tRNA synthetase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC000629
• Gene Symbol: DARS
• Gene ID (NCBI): 1615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.