CoraLite® Plus 488-conjugated DACH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

DACH1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6D2

Cat no : CL488-60082

Synonyms

Dachshund-1, DACH, 3B6D2

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-60082

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

• 

  IF Staining of mouse brain using CL488-60082

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60082 targets DACH1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DACH1 fusion protein Ag4474
• Full Name: dachshund homolog 1 (Drosophila)
• Calculated Molecular Weight: 79 kDa
• GenBank Accession Number: BC021219
• Gene Symbol: DACH1
• Gene ID (NCBI): 1602
• RRID: AB_2883099
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110 kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.