CoraLite® Plus 647-conjugated Cytokeratin 19 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 19 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14965

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL647-14965

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-14965) at dilution of 1:200.

• 

  FC experiment of HeLa using CL647-14965

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cytokeratin 19 (CL647-14965) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-14965 targets Cytokeratin 19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag6830
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40 kDa, 46 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.