Cytokeratin 19 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14965-1-AP

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

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Validation Data Gallery

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  WB analysis of MCF-7 using 14965-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14965-1-AP

  WB result of KRT19 antibody (14965-1-AP, 1:50,000) with si-Control and si-KRT19 transfected HeLa cells.

• 

  WB analysis of HepG2 using 14965-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14965-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 14965-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  FC experiment of MCF-7 using 14965-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug Cytokeratin 19 Polyclonal antibody (14965-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells, HepG2 cells, BxPC-3 cells
• Positive IHC detected in: human colon tissue, human liver cancer tissue, human cervical cancer tissue, human brown disease, mouse colon tissue, rat colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A431 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

14965-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag6830
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40 kDa, 46 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• RRID: AB_2133324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.

Publications

Species	Application	Title
mouse	IF	Gastroenterology; ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.; Authors - Weiguo Fan
mouse	IF	Hepatology; Cholestasis-induced adaptive remodeling of interlobular bile ducts.; Authors - Nachiket Vartak
monkey	IHC,IF	Int J Biol Sci; Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates; Authors - Hongmei Li
human	IHC	Cell Rep; In Vivo Functional Platform Targeting Patient-Derived Xenografts Identifies WDR5-Myc Association as a Critical Determinant of Pancreatic Cancer.; Authors - Alessandro Carugo
mouse	IHC	Arch Toxicol; Galunisertib modifies the liver fibrotic composition in the Abcb4Ko mouse model.; Authors - Seddik Hammad
mouse	IF	Front Cell Dev Biol; Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice; Authors - Stefanie Bobe

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