Cytokeratin 18 Monoclonal antibody, PBS Only (Detector)

Cytokeratin 18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat no : 66187-1-PBS

Synonyms

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

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Validation Data Gallery

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  WB analysis using 66187-1-Ig (same clone as 66187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skin using 66187-1-Ig (same clone as 66187-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:400 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 18 (66187-1-Ig, Clone:1G11C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50238-1

  Cytometric bead array standard curve of MP50238-1, KRT18 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66187-2-PBS. Detection antibody: 66187-1-PBS. Standard:Ag1260. Range: 0.391-100 ng/mL.

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Product Information

66187-1-PBS targets Cytokeratin 18 as part of a matched antibody pair:

MP50238-1: 66187-2-PBS capture and 66187-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Full Name: keratin 18
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000180
• Gene Symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.