Cytokeratin 14 Recombinant antibody

Cytokeratin 14 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H22

Cat no : 83379-1-RR

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

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Validation Data Gallery

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  WB analysis of rat skin using 83379-1-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83379-1-RR (KRT14 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83379-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83379-1-RR against Human Cytokeratin 14 were performed. The affinity constant is 43.1 nM.

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Tested Applications

• Positive WB detected in: rat skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83379-1-RR is Cytokeratin 14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag0188
• Full Name: keratin 14
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC002690
• Gene Symbol: Cytokeratin 14
• Gene ID (NCBI): 3861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.