Cytokeratin 14 Recombinant antibody, PBS Only

Cytokeratin 14 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L19

Cat no : 82824-1-PBS

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Validation Data Gallery

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  WB analysis using 82824-1-RR (same clone as 82824-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 82824-1-RR (same clone as 82824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 82824-1-RR (same clone as 82824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 82824-1-RR (same clone as 82824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 82824-1-RR (same clone as 82824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82824-1-PBS targets Cytokeratin 14 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Full Name: keratin 14
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC002690
• Gene Symbol: KRT14
• Gene ID (NCBI): 3861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.