Cytokeratin 14 Recombinant antibody

• Featured Product
• KD/KO Validated

Cytokeratin 14 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1L19

Cat no : 82824-1-RR

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

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Validation Data Gallery

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  WB analysis using 82824-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 82824-1-RR

  WB result of Cytokeratin 14 antibody (82824-1-RR; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 14 transfected A431 cells.

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  IHC staining of human oesophagus cancer using 82824-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 82824-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 82824-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 82824-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human bowens disease using 82824-1-RR

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human bowens disease using 82824-1-RR

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 82824-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 14 antibody (82824-1-RR, Clone: 1L19 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  Affinity and Kinetic Characterization of 82824-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82824-1-RR against Human Cytokeratin 14 were performed. The affinity constant is 0.989 nM.

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Tested Applications

• Positive WB detected in: mouse skin tissue, A431 cells, rat skin tissue
• Positive IHC detected in: human oesophagus cancer tissue, mouse skin tissue, Human bowens disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82824-1-RR targets Cytokeratin 14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Full Name: keratin 14
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC002690
• Gene Symbol: Cytokeratin 14
• Gene ID (NCBI): 3861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.