Cytochrome c Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Cytochrome c Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat no : 83276-1-PBS

Synonyms

CYCS, CYC, 230503E10

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Validation Data Gallery

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  WB analysis using 83276-1-RR (same clone as 83276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 83276-1-RR (same clone as 83276-1-PBS)

  WB result of Cytochrome c antibody (83276-1-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83276-1-RR (same clone as 83276-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (83276-1-RR, Clone: 230503E10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83276-1-RR (same clone as 83276-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83276-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83276-1-RR against Human Cytochrome c were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83276-1-PBS is Cytochrome c Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytochrome c fusion protein Ag1455
• Full Name: cytochrome c, somatic
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12-15 kDa
• GenBank Accession Number: BC009578
• Gene Symbol: Cytochrome c
• Gene ID (NCBI): 54205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.