Cytochrome c Recombinant antibody, PBS Only

Cytochrome c Recombinant Antibody for WB, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat no : 83276-1-PBS

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

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Validation Data Gallery

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  WB analysis using 83276-1-RR (same clone as 83276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83276-1-RR (same clone as 83276-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83276-1-PBS is Cytochrome c Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytochrome c fusion protein Ag1455
• Full Name: cytochrome c, somatic
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: BC009578
• Gene Symbol: CYCS
• Gene ID (NCBI): 54205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.