Cytochrome c Recombinant antibody

Cytochrome c Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat no : 83276-1-RR

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

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Validation Data Gallery

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  WB analysis using 83276-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83276-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, mouse kidney tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83276-1-RR is Cytochrome c Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytochrome c fusion protein Ag1455
• Full Name: cytochrome c, somatic
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12-15 kDa
• GenBank Accession Number: BC009578
• Gene Symbol: CYCS
• Gene ID (NCBI): 54205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.