Cyclin E1 Recombinant antibody, PBS Only

Cyclin E1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240136D4

Cat no : 82700-10-PBS

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

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Validation Data Gallery

• 

  WB analysis of HT-29 using 82700-10-RR (same clone as 82700-10-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

• 

  WB analysis using 82700-10-RR (same clone as 82700-10-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 82700-10-RR (same clone as 82700-10-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

• 

  WB analysis using 82700-10-RR (same clone as 82700-10-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 82700-10-RR (same clone as 82700-10-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82700-10-RR (same clone as 82700-10-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82700-10-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-10-RR against Human Cyclin E were performed. The affinity constant is 0.564 nM.

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Product Information

The immunogen of 82700-10-PBS is Cyclin E1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cyclin E1 fusion protein Ag2110
• Full Name: cyclin E1
• Calculated Molecular Weight: 410 aa, 47 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC035498
• Gene Symbol: Cyclin E1
• Gene ID (NCBI): 898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).