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Cyclin E Recombinant antibody

Cyclin E Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240136D4

Cat no : 82700-10-RR

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E1, CCNE

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using Cyclin E Recombinant antibody (82700-10-RR)

    WB analysis of HT-29 using 82700-10-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Cyclin E Recombinant antibody (82700-10-RR)

    WB analysis using 82700-10-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Cyclin E Recombinant antibody (82700-10-RR)

    WB analysis of rat heart using 82700-10-RR

    rat heart tissue were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Cyclin E Recombinant antibody (82700-10-RR)

    WB analysis using 82700-10-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cyclin E Recombinant antibody (82700-10-RR)

    FC experiment of MCF-7 using 82700-10-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using Cyclin E Recombinant antibody (82700-10-RR)

    FC experiment of HeLa using 82700-10-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82700-10-RR

    Affinity and Kinetic Characterization of 82700-10-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-10-RR against Human Cyclin E were performed. The affinity constant is 0.564 nM.

Tested Applications

Positive WB detected inHT-29 cells, MCF-7 cells, rat heart tissue, HuH-7 cells
Positive FC (Intra) detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82700-10-RR is Cyclin E Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin E fusion protein Ag2110
Full Name cyclin E1
Calculated Molecular Weight 410 aa, 47 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC035498
Gene Symbol Cyclin E1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

Protocols

Product Specific Protocols
WB protocol for Cyclin E antibody 82700-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols