CoraLite® Plus 488-conjugated Complement factor B Monoclonal antibody

Complement factor B Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

8D2B6

Cat no : CL488-66154

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

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Validation Data Gallery

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  IF Staining of human liver using CL488-66154

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Complement factor B antibody (CL488-66154, Clone: 8D2B6 ) at dilution of 1:200.

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  IF Staining of human liver using CL488-66154

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Complement factor B antibody (CL488-66154, Clone: 8D2B6 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66154 targets Complement factor B in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Complement factor B fusion protein Ag19107
• Full Name: complement factor B
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC007990
• Gene Symbol: Complement factor B
• Gene ID (NCBI): 629
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.