Complement factor B Monoclonal antibody
Complement factor B Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
8D2B6
Cat no : 66154-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human blood tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 66154-1-Ig is Complement factor B Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Immunogen | Complement factor B fusion protein Ag19107 |
Full Name | complement factor B |
Calculated Molecular Weight | 86 kDa |
Observed Molecular Weight | 93 kDa |
GenBank Accession Number | BC007990 |
Gene Symbol | Complement factor B |
Gene ID (NCBI) | 629 |
RRID | AB_2881550 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Thiophilic affinity chromatograph |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
Protocols
Product Specific Protocols | |
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WB protocol for Complement factor B antibody 66154-1-Ig | Download protocol |
IHC protocol for Complement factor B antibody 66154-1-Ig | Download protocol |
IF protocol for Complement factor B antibody 66154-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |