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Complement factor B Monoclonal antibody

Complement factor B Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

8D2B6

Cat no : 66154-1-Ig

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Complement factor B Monoclonal antibody (66154-1-Ig)

    WB analysis of human plasma using 66154-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66154-1-Ig is Complement factor B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag19107
Full Name complement factor B
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
RRIDAB_2881550
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Protocols

Product Specific Protocols
WB protocol for Complement factor B antibody 66154-1-IgDownload protocol
IHC protocol for Complement factor B antibody 66154-1-IgDownload protocol
IF protocol for Complement factor B antibody 66154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols