Complement factor B Monoclonal antibody, PBS Only

Complement factor B Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8D2B6

Cat no : 66154-1-PBS

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

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Validation Data Gallery

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  WB analysis of human plasma using 66154-1-Ig (same clone as 66154-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66154-1-PBS targets Complement factor B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Complement factor B fusion protein Ag19107
• Full Name: complement factor B
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC007990
• Gene Symbol: CFB
• Gene ID (NCBI): 629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.