CoraLite®594-conjugated Collagen Type I Monoclonal antibody
Collagen Type I Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E9A7
Cat no : CL594-67288
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human colon cancer tissue, human breast cancer tissue, mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL594-67288 targets Collagen Type I in IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | collagen, type I, alpha 1 |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_000088 |
| Gene Symbol | COL1A1 |
| Gene ID (NCBI) | 1277 |
| RRID | AB_2920107 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Collagen Type I antibody CL594-67288 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Areli (Verified Customer) (03-14-2023) | I would use a higher dilution next time as the signal was high.
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