Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A7

Cat no : 67288-1-Ig

Synonyms

COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen



Tested Applications

Positive WB detected inpig colon tissue, human cervical cancer tissue, human placenta tissue, pig lung tissue, pig skin tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67288-1-Ig targets Collagen Type I in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, pig, rabbit, zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name collagen, type I, alpha 1
Calculated Molecular Weight139 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberNM_000088
Gene Symbol COL1A1
Gene ID (NCBI) 1277
RRIDAB_2882554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).

Protocols

Product Specific Protocols
WB protocol for Collagen Type I antibody 67288-1-IgDownload protocol
IHC protocol for Collagen Type I antibody 67288-1-IgDownload protocol
IF protocol for Collagen Type I antibody 67288-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Adv Mater

Biodegradable Piezoelectric-Conductive Integrated Hydrogel Scaffold for Repair of Osteochondral Defects

Authors - Dingge Liu
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
humanIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi
humanIHC

Adv Sci (Weinh)

Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner

Authors - Hao Sun
WB,IHC

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
WB

Eur Respir J

Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.

Authors - Shengren Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Niky (Verified Customer) (02-26-2024)

Arrived very quickly on ice. However, didnt work for my tissue type

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: endometrial lesion
FH

Udesh (Verified Customer) (12-22-2023)

Worked well for IF at 1:400

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: BM-MSCs
FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: human fibroblasts
FH

Ren Jie (Verified Customer) (06-22-2022)

Does not work for pepsin digested human collagen I

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Purified protein
FH

Hala (Verified Customer) (02-26-2021)

very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
FH

Hala (Verified Customer) (02-23-2021)

very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000