CoraLite® Plus 647-conjugated Chromogranin A Monoclonal antibody
Chromogranin A Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3H11C11
Cat no : CL647-60135
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | PC-12 cells, SH-SY5Y cells |
Positive FC (Intra) detected in | Neuro-2a cells, SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-60135 targets Chromogranin A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Chromogranin A fusion protein Ag0807 |
Full Name | chromogranin A (parathyroid secretory protein 1) |
Calculated Molecular Weight | 51 kDa |
GenBank Accession Number | BC006459 |
Gene Symbol | Chromogranin A |
Gene ID (NCBI) | 1113 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 Chromogranin A antibody CL647-60135 | Download protocol |
FC protocol for CL Plus 647 Chromogranin A antibody CL647-60135 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |