CoraLite® Plus 488-conjugated Chromogranin A Polyclonal antibody

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  IF Staining of mouse pancreas using CL488-10529

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

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  IF Staining of mouse pancreas using CL488-10529

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

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  IF Staining of Neuro-2a using CL488-10529

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

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  FC experiment of Neuro-2a using CL488-10529

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Chromogranin A (CL488-10529) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Chromogranin A Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Cat No. CL488-10529

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

CHGA, CGA, ER-37, EA-92, Chromogranin-A

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Tested Applications

• Positive IF-P detected in: mouse pancreas tissue
• Positive IF/ICC detected in: Neuro-2a cells
• Positive FC (Intra) detected in: Neuro-2a cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-10529 targets Chromogranin A in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Chromogranin A fusion protein Ag0807
• Full Name: chromogranin A (parathyroid secretory protein 1)
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: BC006459
• Gene Symbol: Chromogranin A
• Gene ID (NCBI): 1113
• RRID: AB_2883077
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10645
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publications

Species	Application	Title
mouse	IF	bioRxiv; Pathobiont-triggered induction of epithelial IDO1 drives regional susceptibility to Inflammatory Bowel Disease; Authors - Paige N Spencer