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Chk1 Recombinant antibody, PBS Only

Chk1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240618E11

Cat no : 84905-1-PBS

Synonyms

CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chk1 Recombinant antibody (84905-1-RR)

    WB analysis using 84905-1-RR (same clone as 84905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84905-1-RR (CHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Chk1 Recombinant antibody (84905-1-RR)

    IF Staining of HeLa using 84905-1-RR (same clone as 84905-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHK1 antibody (84905-1-RR, Clone: 240618E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

Product Information

84905-1-PBS targets Chk1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Chk1 fusion protein Ag22993
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC004202
Gene Symbol Chk1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.