Chk1 Recombinant antibody, PBS Only

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  WB analysis using 84905-1-RR (same clone as 84905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84905-1-RR (CHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84905-1-RR (same clone as 84905-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHK1 antibody (84905-1-RR, Clone: 240618E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 84905-1-RR (same clone as 84905-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Chk1 Recombinant antibody (84905-1-RR, Clone:240618E11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

Chk1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84905-1-PBS

Clone No.240618E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1

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Product Information

84905-1-PBS targets Chk1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Chk1 fusion protein Ag22993
• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC004202
• Gene Symbol: Chk1
• Gene ID (NCBI): 1111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O14757
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.