CoraLite® Plus 488-conjugated CD68 Polyclonal antibody

CD68 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28058

Synonyms

Cd68, CD68 antigen, gp110, Scard1

---

Validation Data Gallery

• 

  IF Staining of mouse spleen using CL488-28058

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 Cd68 antibody (CL488-28058) at dilution of 1:200.

• 

  IF Staining of mouse spleen using CL488-28058

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 Cd68 antibody (CL488-28058) at dilution of 1:200.

---

Tested Applications

• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28058 targets CD68 in IF-P applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD68 fusion protein Ag27827
• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001291058
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.