CD68 Polyclonal antibody

CD68 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


mouse and More (3)





Cat no : 28058-1-AP


Cd68, CD68 antigen, gp110, Scard1

Tested Applications

Positive WB detected inRAW 264.7 cells, mouse spleen tissue
Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Positive FC detected inRAW 264.7 cells

Recommended dilution

Western Blot (WB)WB : 1:600-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28058-1-AP targets CD68 in WB, IF, FC, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag27827
Full Name CD68 antigen
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberNM_001291058
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.


Product Specific Protocols
WB protocol for CD68 antibody 28058-1-APDownload protocol
IHC protocol for CD68 antibody 28058-1-APDownload protocol
IF protocol for CD68 antibody 28058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Alexandru (Verified Customer) (11-13-2023)

Great antibody, worked well in my experiments.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse ventricle

Amelie (Verified Customer) (09-09-2019)

sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse adrenal gland
CD68 Antibody Immunofluorescence, validation (1:1000 dilution) in mouse adrenal gland (Cat no:28058-1-AP)