CoraLite®594-conjugated Caspase 8/p43/p18 Monoclonal antibody

• Featured Product
• KD/KO Validated

Caspase 8/p43/p18 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B9H8

Cat no : CL594-66093

Synonyms

ALPS2B, Apoptotic cysteine protease, Apoptotic protease Mch 5, CAP4, Casp 8, CASP8, Caspase 8, Caspase8, FADD like ICE, FLICE, FLJ17672, ICE like apoptotic protease 5, MACH, MCH5, MORT1 associated ced 3 homolog

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66093

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66093 (Caspase 8/p43/p18 antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL594-66093

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Caspase 8/p43/p18 antibody (CL594-66093, Clone: 2B9H8 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66093 is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 8/p43/p18 fusion protein Ag20524
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 53-57 kDa, 32-45 kDa, 18 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: Caspase 8
• Gene ID (NCBI): 841
• RRID: AB_2883508
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.