CoraLite® Plus 488-conjugated Caspase 8/P43/P18 Polyclonal antibody

• 

  IF Staining of HepG2 using CL488-13423

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Caspase 8/P43/P18 antibody (CL488-13423) at dilution of 1:100, CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using CL488-13423

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Caspase 8/P43/P18 Polyclonal Antibody (CL488-13423)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

Caspase 8/P43/P18 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-13423

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13423 targets Caspase 8/P43/P18 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 8/P43/P18 fusion protein Ag4214
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 53-57 kDa, 32-45 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: Caspase 8
• Gene ID (NCBI): 841
• RRID: AB_3672564
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14790
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.