CoraLite®594-conjugated Calsequestrin 2 Monoclonal antibody

Calsequestrin 2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C10A1

Cat no : CL594-66419

Synonyms

Calsequestrin 2, CASQ2, FLJ26321, FLJ93514, PDIB2

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-66419

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Calsequestrin 2 antibody (CL594-66419, Clone: 1C10A1 ) at dilution of 1:200.

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  IF Staining of mouse heart using CL594-66419

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Calsequestrin 2 antibody (CL594-66419, Clone: 1C10A1 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66419 targets Calsequestrin 2 in IF-P applications and shows reactivity with human, rat, pig, mouse samples.

• Tested Reactivity: human, rat, pig, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calsequestrin 2 fusion protein Ag13246
• Full Name: calsequestrin 2 (cardiac muscle)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022288
• Gene Symbol: Calsequestrin 2
• Gene ID (NCBI): 845
• RRID: AB_2919988
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.