Calsequestrin 2 Monoclonal antibody

Calsequestrin 2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C10A1

Cat no : 66419-1-Ig

Synonyms

Calsequestrin 2, CASQ2, PDIB2

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Validation Data Gallery

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  WB analysis using 66419-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66419-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 66419-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 66419-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66419-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human heart using 66419-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens).

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  IF Staining of mouse heart using 66419-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 66419-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, pig skeletal muscle tissue, rat heart tissue, mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66419-1-Ig targets Calsequestrin 2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

• Tested Reactivity: human, rat, pig, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calsequestrin 2 fusion protein Ag13246
• Full Name: calsequestrin 2 (cardiac muscle)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022288
• Gene Symbol: Calsequestrin 2
• Gene ID (NCBI): 845
• RRID: AB_2881791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

Publications

Species	Application	Title
human	IHC	J Neuropathol Exp Neurol; Morphological Alterations of the Sarcotubular System in Permanent Myopathy of Hereditary Hypokalemic Periodic Paralysis with a Mutation in the CACNA1S Gene.; Authors - Takamura Nagasaka