Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 66419-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 66419-1-Ig
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 66419-1-Ig
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 66419-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens).
IHC staining of human heart using 66419-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens).
IF Staining of mouse heart using 66419-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
pig heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, pig skeletal muscle tissue, rat heart tissue, mouse heart tissue
Positive IHC detected in
human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse heart tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
Protocols
Product Specific Protocols
WB protocol for Calsequestrin 2 antibody 66419-1-Ig
Morphological Alterations of the Sarcotubular System in Permanent Myopathy of Hereditary Hypokalemic Periodic Paralysis with a Mutation in the CACNA1S Gene.
Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 66419-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 66419-1-Ig
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 66419-1-Ig
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 66419-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens).
IHC staining of human heart using 66419-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens).
IF-P Figures
IF Staining of mouse heart using 66419-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse heart using 66419-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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