Calpastatin Monoclonal antibody, PBS Only

Calpastatin Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12A2

Cat no : 67107-1-PBS

Synonyms

BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component

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Validation Data Gallery

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  WB analysis of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67107-1-Ig (same clone as 67107-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67107-1-Ig (same clone as 67107-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67107-1-Ig (same clone as 67107-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67107-1-Ig (same clone as 67107-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 67107-1-Ig (same clone as 67107-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67107-1-PBS targets Calpastatin in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calpastatin fusion protein Ag28669
• Full Name: calpastatin
• Calculated Molecular Weight: 667 aa, 72 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC013579
• Gene Symbol: Calpastatin
• Gene ID (NCBI): 831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.