Calpastatin Monoclonal antibody

Calpastatin Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1G12A2

Cat no : 67107-1-Ig

Synonyms

CAST, Sperm BS-17 component, Calpain inhibitor, 1G12A2

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Validation Data Gallery

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  WB analysis of HeLa using 67107-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67107-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67107-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67107-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67107-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67107-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67107-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 67107-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Calpastatin Monoclonal antibody (67107-1-Ig, Clone:1G12A2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, LNCaP cells, MCF-7 cells, THP-1 cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67107-1-Ig targets Calpastatin in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calpastatin fusion protein Ag28669
• Full Name: calpastatin
• Calculated Molecular Weight: 667 aa, 72 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC013579
• Gene Symbol: Calpastatin
• Gene ID (NCBI): 831
• RRID: AB_2882411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.; Authors - Fengming Shen
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang