CoraLite®594-conjugated Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for WB, IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A2C6

Cat no : CL594-66903

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

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Validation Data Gallery

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  WB analysis using CL594-66903

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66903 (Calnexin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using CL594-66903

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Calnexin antibody (CL594-66903, Clone: 2A2C6 ) at dilution of 1:200.

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  IF Staining of SKOV-3 using CL594-66903

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®594 Calnexin antibody (CL594-66903, Clone: 2A2C6 ) at dilution of 1:200.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66903 targets Calnexin in WB, IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calnexin fusion protein Ag25535
• Full Name: calnexin
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC003552
• Gene Symbol: Calnexin
• Gene ID (NCBI): 821
• RRID: AB_2920054
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.