CoraLite® Plus 488-conjugated Caldesmon Polyclonal antibody

• Featured Product
• KD/KO Validated

Caldesmon Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20887

Synonyms

CALD1, CDM, caldesmon 1, CAD

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Validation Data Gallery

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  IF Staining of mouse heart using CL488-20887

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Caldesmon antibody (CL488-20887) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using CL488-20887

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Caldesmon antibody (CL488-20887) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using CL488-20887

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Caldesmon antibody (CL488-20887) at dilution of 1:200, CL594-phalloidin (red).

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Tested Applications

• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20887 targets Caldesmon in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caldesmon fusion protein Ag13676
• Full Name: caldesmon 1
• Calculated Molecular Weight: 538 aa, 63 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC040354
• Gene Symbol: CALD1
• Gene ID (NCBI): 800
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).