CoraLite® Plus 488-conjugated CYP2E1-Specific Monoclonal antibody

CYP2E1-Specific Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig, human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F12G7

Cat no : CL488-67263

Synonyms

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

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Validation Data Gallery

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  IF Staining of mouse liver using CL488-67263

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

• 

  IF Staining of mouse liver using CL488-67263

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67263 targets CYP2E1-Specific in IF-P applications and shows reactivity with mouse, rat, pig, human samples.

• Tested Reactivity: mouse, rat, pig, human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: NM_000773
• Gene Symbol: CYP2E1
• Gene ID (NCBI): 1571
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.