CYP2E1-Specific Monoclonal antibody

CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig, human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1F12G7

Cat no : 67263-1-Ig

Synonyms

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

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Validation Data Gallery

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  WB analysis of pig liver using 67263-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HaCaT using 67263-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67263-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 67263-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 67263-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP2E1-Specific antibody (67263-1-Ig, Clone: 1F12G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig liver tissue, rat liver tissue, mouse liver tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

67263-1-Ig targets CYP2E1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat, pig, human samples.

• Tested Reactivity: mouse, rat, pig, human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: NM_000773
• Gene Symbol: CYP2E1
• Gene ID (NCBI): 1571
• RRID: AB_2882534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Publications

Species	Application	Title
mouse	WB	Biomed Pharmacother; The effects of scoparone on alcohol and high-fat diet-induced liver injury revealed by RNA sequencing; Authors - Min Wei
mouse	WB,IHC	Front Pharmacol; Methyl 6-O-cinnamoyl-α-d-glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway.; Authors - Qianqian Xu
mouse	WB	Med Sci Monit; Comparison of the Effects of Intraperitoneal Injection with Carbon Tetrachloride on Acute Liver Toxicity in Male and Female Kunming Mice; Authors - Huan Yang
mouse	WB	Nutrients; Daucosterol Alleviates Alcohol−Induced Hepatic Injury and Inflammation through P38/NF−κB/NLRP3 Inflammasome Pathway; Authors - Feng Zhang