CYP2E1-Specific Monoclonal antibody

CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig, human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G7

Cat no : 67263-1-Ig

Synonyms

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E



Tested Applications

Positive WB detected inpig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67263-1-Ig targets CYP2E1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat, pig, human samples.

Tested Reactivity mouse, rat, pig, human
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 2, subfamily E, polypeptide 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberNM_000773
Gene Symbol CYP2E1
Gene ID (NCBI) 1571
RRIDAB_2882534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Protocols

Product Specific Protocols
WB protocol for CYP2E1-Specific antibody 67263-1-IgDownload protocol
IHC protocol for CYP2E1-Specific antibody 67263-1-IgDownload protocol
IF protocol for CYP2E1-Specific antibody 67263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

The effects of scoparone on alcohol and high-fat diet-induced liver injury revealed by RNA sequencing

Authors - Min Wei
mouseWB,IHC

Front Pharmacol

Methyl 6-O-cinnamoyl-α-d-glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway.

Authors - Qianqian Xu
mouseWB

Med Sci Monit

Comparison of the Effects of Intraperitoneal Injection with Carbon Tetrachloride on Acute Liver Toxicity in Male and Female Kunming Mice

Authors - Huan Yang
mouseWB

Nutrients

Daucosterol Alleviates Alcohol−Induced Hepatic Injury and Inflammation through P38/NF−κB/NLRP3 Inflammasome Pathway

Authors - Feng Zhang