CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP1A1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13241

Synonyms

Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR

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Validation Data Gallery

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  FC experiment of HepG2 using CL647-13241

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody (CL647-13241)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13241 targets CYP1A1 in FC (Intra) applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP1A1 fusion protein Ag3990
• Full Name: cytochrome P450, family 1, subfamily A, polypeptide 1
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC023019
• Gene Symbol: CYP1A1
• Gene ID (NCBI): 1543
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.