CoraLite®594-conjugated CYB5R3 Monoclonal antibody

• Featured Product
• KD/KO Validated

CYB5R3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

9C4D10

Cat no : CL594-66022

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66022

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66022 (CYB5R3 antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL594-66022

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CYB5R3 antibody (CL594-66022, Clone: 9C4D10 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66022 targets CYB5R3 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYB5R3 fusion protein Ag18096
• Full Name: cytochrome b5 reductase 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004821
• Gene Symbol: CYB5R3
• Gene ID (NCBI): 1727
• RRID: AB_2883481
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.