CYB5R3 Monoclonal antibody

• Featured Product
• KD/KO Validated

CYB5R3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

9C4D10

Cat no : 66022-1-Ig

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

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Validation Data Gallery

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  WB analysis of HeLa using 66022-1-Ig

  WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells.

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  WB analysis of HeLa using 66022-1-Ig

  Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 66022-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66022-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66022-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66022-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66022-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66022-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66022-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66022-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66022-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human heart tissue, human plasma tissue, HepG2 cells, L02 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66022-1-Ig targets CYB5R3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYB5R3 fusion protein Ag18096
• Full Name: cytochrome b5 reductase 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004821
• Gene Symbol: CYB5R3
• Gene ID (NCBI): 1727
• RRID: AB_11045662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.