CYB5R3 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

  WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

  Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66022-1-Ig (same clone as 66022-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66022-1-Ig (same clone as 66022-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66022-1-Ig (same clone as 66022-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66022-1-Ig (same clone as 66022-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66022-1-Ig (same clone as 66022-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66022-1-Ig (same clone as 66022-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CYB5R3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66022-1-PBS

Clone No.9C4D10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

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Product Information

66022-1-PBS targets CYB5R3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYB5R3 fusion protein Ag18096
• Full Name: cytochrome b5 reductase 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004821
• Gene Symbol: CYB5R3
• Gene ID (NCBI): 1727
• RRID: AB_11045662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00387
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.