Filter: AllWBIHCIF-P

WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)
MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 60065-1-Ig (same clone as 60065-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 60065-1-Ig (same clone as 60065-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
WB analysis of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)
U-937 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 60065-1-Ig (same clone as 60065-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CXCR3B-specific antibody (60065-1-Ig, Clone: 1B2D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CXCR3B-specific antibody (60065-1-Ig, Clone: 1B2D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CXCR3B-specific Monoclonal antibody, PBS Only

CXCR3B-specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60065-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CXCR3, CMKAR3, CKR-L2, CKR L2, CD183

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60065-1-PBS targets CXCR3B-specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	chemokine (C-X-C motif) receptor 3
Calculated Molecular Weight	46 kDa
Observed Molecular Weight	43 kDa
GenBank Accession Number	AF469635
Gene Symbol	CXCR3
Gene ID (NCBI)	2833
RRID	AB_2292376
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P49682
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.